Pollen Germination: Terms Explained
Question
Germination of pollen grain on stigma is:
(1) ex-situ
(2) in-situ
(3) in-vivo
(4) More than one correct
(1) ex-situ
(2) in-situ
(3) in-vivo
(4) More than one correct
Answer
✅ Correct Answer: (2) in-situ and (3) in-vivo
➡️ So, option (4) “More than one correct” is the right choice.
➡️ So, option (4) “More than one correct” is the right choice.
Explanation
Germination on stigma happens inside the flower (living plant) ➡️ In Vivo.
And stigma is its natural site ➡️ In Situ.
So both are correct ✅✅.
And stigma is its natural site ➡️ In Situ.
So both are correct ✅✅.
Your Doubt, as asked in Class ?
Why ex-situ is not correct? Pollen grain moves from where it is produced (anther) to somewhere else (stigma). Isn’t this ex-situ?
Explanation ✅
Excellent observation by you but we often get confused here.
🔸 Pollen grain is produced in the anther.
🔸 It germinates on the stigma.
Now your thought: Pollen moves → so is this ex-situ?
❌ Answer: No. Because in biology, in situ refers to processes happening in their natural site of function, not the site of origin.
➡️ So, germination on stigma is still in situ.
🔸 Pollen grain is produced in the anther.
🔸 It germinates on the stigma.
Now your thought: Pollen moves → so is this ex-situ?
❌ Answer: No. Because in biology, in situ refers to processes happening in their natural site of function, not the site of origin.
➡️ So, germination on stigma is still in situ.
Terms Definitions
In Vivo
Process occurs inside a living organism.
In Situ
Process occurs at its natural or original site.
Ex Situ
Process occurs outside its natural site in an artificial environment.
In Vitro
Process occurs outside organism in glass/lab conditions.
Future Ready Information
Process / Location | In Vivo | In Situ | Ex Situ | In Vitro |
---|---|---|---|---|
Pollen formation inside anther | ✅ Yes | ✅ Yes | ❌ No | ❌ No |
Pollen germination on stigma (natural) | ✅ Yes | ✅ Yes | ❌ No | ❌ No |
Pollen germination in lab (on medium) | ❌ No | ❌ No | ✅ Yes | ✅ Yes |
Pollination (transfer anther to stigma) | 🚫 Movement | 🚫 Movement | 🚫 Movement | 🚫 Movement |
Practice Questions
Q1
Where does in-vitro pollen germination occur?
✅ Answer: Outside organism in lab.
🔎 Explanation: In vitro means “in glass”, so it’s done in petri dishes or lab medium.
✅ Answer: Outside organism in lab.
🔎 Explanation: In vitro means “in glass”, so it’s done in petri dishes or lab medium.
Q2
Is pollination an example of in situ process?
❌ Answer: No.
🔎 Explanation: Pollination is just movement, not a process of function at natural site.
❌ Answer: No.
🔎 Explanation: Pollination is just movement, not a process of function at natural site.
Q3
Pollen germination on stigma in flower is called?
✅ Answer: In vivo and In situ.
🔎 Explanation: Happens inside plant (in vivo) and at its natural site (in situ).
✅ Answer: In vivo and In situ.
🔎 Explanation: Happens inside plant (in vivo) and at its natural site (in situ).
Q4
Ex situ pollen germination is seen where?
✅ Answer: In laboratory.
🔎 Explanation: Ex situ is outside natural site, so lab medium is ex situ.
✅ Answer: In laboratory.
🔎 Explanation: Ex situ is outside natural site, so lab medium is ex situ.
Q5
Formation of pollen in anther is?
✅ Answer: In vivo and In situ.
🔎 Explanation: Happens inside living plant and in its original site.
✅ Answer: In vivo and In situ.
🔎 Explanation: Happens inside living plant and in its original site.
MCQ Practice Questions
Q1
Where does in-vitro pollen germination occur?
(1) Inside the organism
(2) Outside the organism in a lab
(3) In the natural environment
(4) On the stigma of the flower
✅ Answer: (2) Outside the organism in a lab
🔎 Explanation: In vitro means “in glass”, meaning it happens outside the organism, typically in lab conditions.
(1) Inside the organism
(2) Outside the organism in a lab
(3) In the natural environment
(4) On the stigma of the flower
✅ Answer: (2) Outside the organism in a lab
🔎 Explanation: In vitro means “in glass”, meaning it happens outside the organism, typically in lab conditions.
Q2
Pollination is an example of which process?
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) In Vitro
✅ Answer: (2) In Situ
🔎 Explanation: Pollination is the transfer of pollen from one flower to another, occurring at the plant’s natural site.
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) In Vitro
✅ Answer: (2) In Situ
🔎 Explanation: Pollination is the transfer of pollen from one flower to another, occurring at the plant’s natural site.
Q3
Pollen germination on stigma is best described as?
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) Both In Vivo and In Situ
✅ Answer: (4) Both In Vivo and In Situ
🔎 Explanation: Germination on stigma happens inside the plant (in vivo) and at the plant’s natural site (in situ).
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) Both In Vivo and In Situ
✅ Answer: (4) Both In Vivo and In Situ
🔎 Explanation: Germination on stigma happens inside the plant (in vivo) and at the plant’s natural site (in situ).
Q4
Ex situ pollen germination can be observed in?
(1) Laboratory
(2) Natural environment
(3) On stigma
(4) Inside anther
✅ Answer: (1) Laboratory
🔎 Explanation: Ex situ means outside the natural site; lab conditions are where this would happen.
(1) Laboratory
(2) Natural environment
(3) On stigma
(4) Inside anther
✅ Answer: (1) Laboratory
🔎 Explanation: Ex situ means outside the natural site; lab conditions are where this would happen.
Q5
Which term describes the formation of pollen in anther?
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) In Vitro
✅ Answer: (1) In Vivo
🔎 Explanation: Pollen formation happens inside the living plant, so it is an example of “In Vivo”.
(1) In Vivo
(2) In Situ
(3) Ex Situ
(4) In Vitro
✅ Answer: (1) In Vivo
🔎 Explanation: Pollen formation happens inside the living plant, so it is an example of “In Vivo”.